Leica DMI4000 B

Multipurpose Fluorescence System

The Leica DMI4000 B automated inverted research microscope is ideal for scanning (short-term) live and fixed cell and tissue cultures. It is equipped with a colour camera for immunohistochemistry and a highly sensitive black and white camera for fluores-cent imaging. Contrast techniques are brightfield, darkfield, phase, modulation, and dif-ferential interference. The device is equipped with four different filter combinations suit-able for a broad range of different fluorochromes (DAPI, GFP, Cy3, Cy5, etc.). Availa-ble objectives range in magnification from 2.5x to 63x, some being optimized for high-resolution fluorescence imaging.

 Cameras Leica DFC425 C digital colour camera
Leica DFC360 FX B&W fluorescence camera
 Lenses N PLAN 2.5x/0.07
N PLAN 5x/0.12
PLAN 20x/ 0.30
HC PL APO 20x/0.70 IMM CORR CS Oil
HCX PL FLUOTAR L 40x/0.60 CORR
HCX PL APO 63x/1.40-0.60 C Oi
 Filters  DAPI (Hoechst, Alexa Fluor 350) FITC (Cy2, GFP, Alexa Fluor 488) TRITC (Cy3, Rhodamine, Alexa Fluor 547) Cy5 (APC, Alexa 633)
 Illumination  Brightfield, darkfield, phase, modulation, differential interference
 Computer  Leica MMAF PC Workstation
 Software  LAS AF, Leica software for fluorescence acquisition LAS V3.6, Leica software for brightfield acquisition
Leica DMI4000 B