Department for BioMedical Research (DBMR)

Microscopes

Nikon Eclipse Ti-E Spinning Disk

Confocal Microscope

The Nikon Eclipse Ti-E Spinning Disk is a high-end inverted microscope system which offers improved acquisition speed and high flexibility in live cell imaging. The Nikon Eclipse Ti-E Spinning Disk has a fast image acquisition integrated perfect focus system (PFS), which eliminates focus drift. As a multi-point confocal system, the Spinning Disk offers a much greater data throughput while capturing data in 2D and 3D at enhanced speed. The incubation chamber can be used for long time-lapse imaging.

 Cameras  IOXN 888 EMCCD
ORCA-Flash
 Objectives  CFI P-Apo 4x Lambda   4x/0.20/20mm
CFI P-Apo 10x Lambda 10x/0.45/4mm
CFI S P-Fluor EXTRA LONG WORKING DISTANCE 20x/0.45/8.2-6.9mm
CFI S P-Fluor EXTRA LONG WORKING DISTANCE 40x/0.60/3.6-2.8mm
CFI P-Fluor Öl 40x/1.30/0.2mm
CFI P-Apo 60x Lambda Öl 60x/1.40/0.13mm
 Filters  DAPI (Hoechst, Alexa Fluor 350), FITC (Cy2, GFP, Alexa Fluor 488), Rhodamine (Cy3, Alexa Fluor 546…), Cy5 (Alexa Fluor 688…)
 Illumination  Brightfield, Fluorescence (LED), Differential interference, Reflected illumination
 Additional equipment  Incubation unit
 Special application  Optimized for microfluidics imaging, application of scripts (Jobs software)
 Computer  Default PC workstation
 Software  NIS-Elements (Nikon), Jobs (Nikon)
 Contact  Dr. Fabian Blank
Selina Steiner
Carlos Wotzkow
 Location  Departement for BioMedical Research
University of Bern
U3 302
Murtenstrasse 50
3008 Bern
Nikon Eclipse Ti-E Spinning Disk