Department for BioMedical Research (DBMR)

Microscopes

Zeiss LSM710

Confocal Laser Scanning Microscope

The Zeiss LSM710 is a laser scanning confocal microscope that incorporates the latest advances in optics, light path efficiency, low noise scan electronics and detectors. This instrument has advanced imaging capabilities offering high sensitivity with superior signal to noise ratio. It allows the performance of automated long-term multi-position life cell experiments. The system is equipped with a motorized X-Y stage, a piezo stage for fast focussing with a temperature, humidity and CO2 controlled live chamber XL incubator. Data is acquired using the latest version of the Zen software. Acquired data can be imported into all of our image analysis platforms as raw files for further analysis.

 Lasers  405nm, 458nm, 488nm, 514nm, 561nm, 633nm
 Objectives Plan-Apochromat 10x/0.3 M27 /a=2.00mm
Plan-Apochromat 20x/0.8 M27 /a=0.55mm
EC Plan-Neofluar 40x/1.30 Oil DIC M27 /a=0.21mm
Plan-Apochromat 60x/1.40 Oil DIC M27
 Detector  3xPMT, 1xT PMT
 Filters  DAPI (Hoechst, Alexa Fluor 350), FITC (Cy2, GFP, Alexa Fluor 488), Rhodamine (Cy3, Alexa Fluor 546…), Far red (Cy5, Cy7,…)
 Illumination  Transmitted light, white light (fluorescence), laser
 Additional equipment
 Computer  Default PC workstation
 Software  ZEN system 2011
 Contact  Dr. Fabian Blank
Selina Steiner
Carlos Wotzkow
 Location  Department for BioMedical Research
University of Bern
U3 302
Murtenstrasse 50
3008 Bern
Zeiss LSM710