The Flow Cytometry and Cell Sorting Facility (FCCS) is a core facility of the Department for BioMedical Research which provides instruments and expertise for experiments using flow cytometry, imaging flow cytometry, and cell sorting. A wide range of applications is supported, including cell phenotyping, cell cycle analysis, antigen quantification, cell viability (including apoptosis), intracellular calcium measurement, mitochondrial mass and activity, oxidative metabolism, membrane potential, endocytosis, cell fusion, and many others, particularly image-based analyses for morphological and spatial information.
We offer a broad range of top-of-the-line flow cytometers to meet your experimental needs. Measurements are performed on a single-cell basis, allowing the simultaneous assessment of up to 50 parameters. Specific cell subsets can be sorted for subsequent in vitro culture, adoptive transfer, or further cell biological and biochemical analyses. Single cells can also be sorted into 96- or 384-well plates for cloning purposes.
Cell sorters are primarily operated by facility staff, while researchers receive training to independently operate the analyzers. In addition, comprehensive introductory courses in flow cytometry are offered on a regular basis.
